Evaluation of antioxidant and anticancer effects of Piper betle L (Piperaceae) leaf extract on MCF-7 cells, and preparation of transdermal patches of the extract

Purpose: To determine the antioxidant and anticancer effects of Piper betle (P. betle) leaf extract on human breast cancer MCF-7 cells, to develop transdermal patches containing extract.
 Methods: The P. was prepared by maceration method, its activity evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Cytotoxicity suppression cell migration (indices activity) were also assessed in cells sulforhodamine B (SRB) wound healing assays, respectively. Transdermal developed casting resultant with regard their physical appearance mechanical properties before after a stability test.
 Results: exhibited half-maximal inhibitory concentration (IC50) 30.0 ± 0.1 µg/mL. It showed cytotoxicity an IC50 114.3 14.9 µg/mL, significantly suppressed at dose 25 Based desirable characteristics, patch base formulations 4.2 % pectin, 0.4 hydroxyl propyl methylcellulose (HPMC), polyvinyl pyrrolidine K-90 (PVP-K90) 3 propylene glycol (PG) selected for incorporation into Conclusion: Leaf exhibits potential anti-breast properties. A 0.03 can be successfully treatment cancer.